Methyltransferase like 3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03290
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human Methyltransferase like 3 (NP_062826.2). MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Methyltransferase like 3 Antibody - Azide and BSA Free (NBP3-03290) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Methyltransferase like 3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Methyltransferase like 3 Antibody - Azide and BSA Free
Western Blot: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] -
Western Blot: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] - Western blot analysis of lysates from wild type (WT) and Methyltransferase like 3 knockdown (KD) 293T cells using Methyltransferase like 3 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] -
Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] - Immunofluorescence analysis of PC-3 cells using Methyltransferase like 3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] -
Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody - Azide and BSA Free [Methyltransferase like 3] - Immunofluorescence analysis of HepG2 cells using Methyltransferase like 3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Applications for Methyltransferase like 3 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Methyltransferase like 3
Alternate Names
adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase, EC 2.1.1.62, IME4, M6A, methyltransferase like 3, Methyltransferase-like protein 3, MGC4336, mRNA m(6)A methyltransferase, MTA70, MT-A70, N6-adenosine-methyltransferase 70 kDa subunit, Spo8
Gene Symbol
METTL3
Additional Methyltransferase like 3 Products
Product Documents for Methyltransferase like 3 Antibody - Azide and BSA Free
Product Specific Notices for Methyltransferase like 3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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