Menin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-399
![Western Blot: Menin Antibody [NB100-399]](https://resources.bio-techne.com/images/products/Menin-Antibody-Western-Blot-NB100-399-img0004.jpg "Western Blot: Menin Antibody [NB100-399]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide, which represented a portion of human Multiple Endocrine Neoplasia 1 encoded within exon 11 (MEN1 LocusLink ID 4221).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Menin Antibody - BSA Free
Western Blot: Menin Antibody [NB100-399]
Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody (lot was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: Menin Antibody [NB100-399]](https://resources.bio-techne.com/images/products/Menin-Antibody-Western-Blot-NB100-399-img0002.jpg "Western Blot: Menin Antibody [NB100-399]")
Western Blot: Menin Antibody [NB100-399]
Western Blot: Menin Antibody [NB100-399] - A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). B. Whole cell lysate from one 10 cm plate of untreated 293T cells. A. Affinity purified rabbit anti-Menin antibody NB100-399 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies NB100-215 (5 mcg/plate), NB100-398(10 mcg/plate). Immunoprecipitated Menin was detected using NB100-215 at 0.1 mcg/ml.![Western Blot: Menin Antibody [NB100-399]](https://resources.bio-techne.com/images/products/Menin-Antibody-Western-Blot-NB100-399-img0003.jpg "Western Blot: Menin Antibody [NB100-399]")
Western Blot: Menin Antibody [NB100-399]
Western Blot: Menin Antibody [NB100-399] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Menin Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:500-1:5000
Application Notes
This antibody may be used for Western Blot, where a doublet band should be seen at ~72 kDa. Suggested working dilutions: * Western Blot - 1:500-1:5,000 Immunoprecipitation - 2 to 10 ug/mg lysate Immunofluorescence - ND *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Menin
Long Name
Multiple Endocrine Neoplasia I
Alternate Names
MEAI, MEN1
Entrez Gene IDs
4221 (Human)
Gene Symbol
MEN1
Additional Menin Products
Product Documents for Menin Antibody - BSA Free
Product Specific Notices for Menin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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