MDM2/HDM2 Antibody (24GB7055)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50875

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NBP3-50875-100ul
NBP3-50875-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 24GB7055

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MDM2/HDM2 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human MDM2/HDM2 (Uniprot: Q00987)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Scientific Data Images for MDM2/HDM2 Antibody (24GB7055)

MDM2/HDM2 Antibody (24GB7055)

Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (24GB7055)[NBP3-50875] -

Immunocytochemical staining of C2C12 cells with MDM2/HDM2 antibody (1:1,000). Nuclei were stained blue with DAPI;MDM2/HDM2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MDM2/HDM2 Antibody (24GB7055)

Flow Cytometry: MDM2/HDM2 Antibody (24GB7055)[NBP3-50875] -

Flow cytometric analysis of MDM2/HDM2expression in C2C12 cells using MDM2/HDM2 antibody (1:2,000). Green, isotype control; red, MDM2.
MDM2/HDM2 Antibody (24GB7055)

Western Blot: MDM2/HDM2 Antibody (24GB7055)[NBP3-50875] -

Western blotting analysis using MDM2/HDM2 antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for MDM2/HDM2 Antibody (24GB7055)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDM2/HDM2

MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Gene Symbol

MDM2

Additional MDM2/HDM2 Products

Product Documents for MDM2/HDM2 Antibody (24GB7055)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MDM2/HDM2 Antibody (24GB7055)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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