MDA5 Antibody (24GB6085)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51004
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB6085
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human MDA5 (Uniprot: Q9BYX4)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MDA5 Antibody (24GB6085)
Immunocytochemistry/ Immunofluorescence: MDA5 Antibody (24GB6085)[NBP3-51004] -
Immunocytochemical staining of HepG2 cells with MDA5 antibody (1:1,000). Nuclei were stained blue with DAPI; MDA5 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.Western Blot: MDA5 Antibody (24GB6085)[NBP3-51004] -
Western blotting analysis using MDA5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Western Blot: MDA5 Antibody (24GB6085)[NBP3-51004] -
Western blotting analysis using MDA5 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for MDA5 Antibody (24GB6085)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IFIH1/MDA5
Long Name
Melanoma Differentiation-Associated Protein 5
Alternate Names
AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1
Gene Symbol
IFIH1
Additional IFIH1/MDA5 Products
Product Documents for MDA5 Antibody (24GB6085)
Product Specific Notices for MDA5 Antibody (24GB6085)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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