MCAF1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84610

Novus Biologicals, part of Bio-Techne
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NBP1-84610
NBP1-84610-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCAF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MCAF1 Antibody - BSA Free (NBP1-84610) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MCAF1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
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Applications for MCAF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-84610 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCAF1

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Alternate Names

activating transcription factor 7 interacting protein, activating transcription factor 7-interacting protein 1, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, FLJ10139, FLJ10688, hAM, MBD1-containing chromatin associated factor, MCAF1AM, MCAFMBD1-containing chromatin-associated factor 1, MMCP 6, MMCP6, MMCP-6, P621

Gene Symbol

ATF7IP

Additional MCAF1 Products

Product Documents for MCAF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCAF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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