Mas Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78444

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Mas Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human MAS1 protein (between residues 75-125)

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34558543). Rabbit reactivity reported in the scientific literature (PMID: 23701246).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mas Antibody - BSA Free

Western Blot: Mas AntibodyBSA Free [NBP1-78444]

Western Blot: Mas AntibodyBSA Free [NBP1-78444]

Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.
Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NBP1-78444]

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NBP1-78444]

Immunocytochemistry/Immunofluorescence: Mas Antibody [NBP1-78444] - Tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Mas Antibody - BSA Free [NBP1-78444]

Immunohistochemistry: Mas Antibody - BSA Free [NBP1-78444]

Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
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Applications for Mas Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

1:100

Western Blot

1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 47 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-78444 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mas

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Long Name

MAS1 Oncogene

Alternate Names

MAS1

Entrez Gene IDs

4142 (Human)

Gene Symbol

MAS1

UniProt

P04201 (Human)

Additional Mas Products

Product Documents for Mas Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mas Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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