MAML3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2128
![Western Blot: MAML3 Antibody [NB100-2128]](https://resources.bio-techne.com/images/products/MAML3-Antibody-Western-Blot-NB100-2128-img0008.jpg "Western Blot: MAML3 Antibody [NB100-2128]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAML3 Antibody - BSA Free
Western Blot: MAML3 Antibody [NB100-2128]
Western Blot: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128]](https://resources.bio-techne.com/images/products/MAML3-Antibody-Immunohistochemistry-NB100-2128-img0009.jpg "Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128]")
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128]
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2128). Detection: DAB![Immunoprecipitation: MAML3 Antibody [NB100-2128]](https://resources.bio-techne.com/images/products/MAML3-Antibody-Immunoprecipitation-NB100-2128-img0010.jpg "Immunoprecipitation: MAML3 Antibody [NB100-2128]")
Immunoprecipitation: MAML3 Antibody [NB100-2128]
Immunoprecipitation: MAML3 Antibody [NB100-2128] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibody NB100-2129. For blotting immunoprecipitated MAML3, NB100-2128 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for MAML3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2- 10 ug/mg of lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MAML3
Alternate Names
CAG/CTG 3, ERDA3, MAM-2, mam-3, mastermind-like 3 (Drosophila), mastermind-like protein 3, polyglutamine rich, trinucleotide repeat containing 3
Entrez Gene IDs
55534 (Human)
Gene Symbol
MAML3
UniProt
Additional MAML3 Products
Product Documents for MAML3 Antibody - BSA Free
Product Specific Notices for MAML3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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