Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11E3

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Malondialdehyde Antibodies »

Product Specifications

Immunogen

Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Malondialdehyde Antibody (11E3) - BSA Free

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367]

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367]

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367]

Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367]

Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry: Malondialdehyde Antibody (11E3) [NBP2-59367]

Immunohistochemistry: Malondialdehyde Antibody (11E3) [NBP2-59367]

Immunohistochemistry: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunohistochemistry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 10X.
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Applications for Malondialdehyde Antibody (11E3) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Malondialdehyde

Alternate Names

3-Propanedial;Malonaldehyde;Malondialdehyde;MDA

Additional Malondialdehyde Products

Product Documents for Malondialdehyde Antibody (11E3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Malondialdehyde Antibody (11E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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