MafA Antibody

Immunocytochemistry/ Immunofluorescence: MafA Antibody [NBP1-00121]

Immunocytochemistry/Immunofluorescence: MafA Antibody [NBP1-00121] - Analysis of MafA in 8-week old mouse pancretic cryosection using anti-MafA antibody (red). Image from verified customer review.

Immunohistochemistry-Paraffin: MafA Antibody [NBP1-00121]

Immunohistochemistry-Paraffin: MafA Antibody [NBP1-00121] - Section of mouse pancreatic islet. Antibody: Affinity purified rabbit anti-MafA (Cat. No. NBP1-00121) used at a dilution of 1:250.

Western Blot: MafA Antibody [NBP1-00121] -

Critical beta-cell factors are reduced upon cytokine treatment in mouse and human islets. A-F. Relative mRNA quantification of various beta-cell transcriptional regulator genes (MafA, Pdx1, Nkx6.1, Ldb1, Isl1, and SSBP3, respectively) from primary mouse islets after 4 h culturing with single cytokines (Tnf alpha, Ifn gamma, IL-1 beta) or a cocktail of each, as compared to PBS vehicle controls (set to 1-fold). 36B4 was used as the housekeeping gene; n = 3–4 for each treatment group. G. Left: LDB1, MAFA, NKX6.1, and ISL1 Western blot with Ins-1 cell extracts after 4 h or overnight treatment with single cytokines, or a cocktail, as compared to control. Actin was included as loading control. Right: Densitometry quantification of 4 h or overnight treated LDB1, MAFA, NKX6.1, or ISL1 Western blot protein levels, normalized to Actin. H. Human islets were treated with a DMSO vehicle or a cytokine cocktail,26,27 then mRNA measured. 18S was used as the housekeeping gene; n = 3. I. Quantification of beta-cell mRNAs from primary mouse islets after siRNA-mediated Ldb1 knockdown using Gapdh as the housekeeping gene; n = 3. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA or Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34968409), licensed under a CC-BY license. Not internally tested by Novus Biologicals.