Skip to main content

Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44246

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-44246

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5263

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q9P0M6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Macro H2A.2 Antibody (CL5263) - Azide and BSA Free (NBP2-61156) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

Macro H2A.2 Antibody (CL5263) - Azide and BSA Free Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Staining of human kidney shows strong nuclear positivity in cells in renal glomeruli and a subset of renal tubules.
Macro H2A.2 Antibody (CL5263) - Azide and BSA Free Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular cells and moderate positivity in stromal cells.
Macro H2A.2 Antibody (CL5263) - Azide and BSA Free Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Immunohistochemistry-Paraffin: Macro H2A.2 Antibody [NBP3-44246]

Staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.

Applications for Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Macro H2A.2

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation

Alternate Names

core histone macro-H2A.2, core histone macroH2A2.2, H2A histone family, member Y2, Histone macroH2A2, MACROH2A2, mH2A2

Gene Symbol

MACROH2A2

Additional Macro H2A.2 Products

Product Documents for Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Macro H2A.2 Antibody (CL5263) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...