M-CSFR/CD115 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84532

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NBP1-84532
NBP1-84532-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all M-CSF R/CD115 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for M-CSFR/CD115 Antibody - BSA Free

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human skeletal muscle shows low positivity in myocytes as expected.
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
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Applications for M-CSFR/CD115 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: M-CSF R/CD115

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Long Name

Macrophage Colony Stimulating Factor Receptor

Alternate Names

c-fms, CD115, CSF1R, M-CSFR, MCSFR

Entrez Gene IDs

1436 (Human)

Gene Symbol

CSF1R

UniProt

P07333 (Human)

Additional M-CSF R/CD115 Products

Product Documents for M-CSFR/CD115 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for M-CSFR/CD115 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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