Lymphotoxin Antibody (135125) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1370CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 135125
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha/ beta
Specificity
Recognizes human Lymphotoxin alpha chains in alpha2 beta1 or alpha1 beta2 trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Applications for Lymphotoxin Antibody (135125) [CoraFluor™ 1]
Application
Recommended Usage
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Lymphotoxin
Lymphotoxin alpha and Lymphotoxin beta are pro-inflammatory TNF superfamily ligands that play important roles in immune system development. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI, TNF RII, HVEM, and TROY. It is required for development of the spleen, lymph nodes, and Peyer’s patches. It also regulates T cell homing to the gut and IgA induction. LT-alpha can additionally form membrane-associated heterotrimers with two copies of LT-beta/TNF-beta on the surface of B, T, LTi, and ILC3 cells. This heterotrimer binds and activates the Lymphotoxin beta R on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV). The heterotrimer promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by IEC.
Alternate Names
LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E
Gene Symbol
LTA
Additional Lymphotoxin Products
Product Documents for Lymphotoxin Antibody (135125) [CoraFluor™ 1]
Product Specific Notices for Lymphotoxin Antibody (135125) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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