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LPAR1/LPA1/EDG-2 Antibody - BSA Free

Catalog # NLS212 | Novus Biologicals a Bio-Techne Brand
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NLS212

Key Product Details

Species Reactivity

Human, Rat, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for LPAR1/LPA1/EDG-2 Antibody - BSA Free

Immunogen

Synthetic 15 amino acid peptide from N-terminal extracellular domain of human LPAR1/LPA1/EDG-2.

Epitope

N-Terminus extracellular domain

Predicted Species

Avian (93%), Bat (93%), Bovine (93%), Canine (93%), Chicken (93%), Equine (93%), Hamster (93%), Mouse (93%), Opossum (93%), Porcine (93%), Rabbit (93%), Sheep (93%). Backed by our 100% Guarantee.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Xenopus (87%), Zebrafish (87%). Rat reactivity reported in (PMID: 23451264).

Localization

Cell Membrane

Specificity

Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53%), ACER3 (47%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for LPAR1/LPA1/EDG-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Skin, Melanoma
Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Brain, Glioblastoma

Applications for LPAR1/LPA1/EDG-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 31546971).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using NLS212 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: LPAR1/LPA1/EDG-2

Edg2, a Lysophospholipid/Lysosphingolipid Receptor, mediates growth factor-like activities such as proliferation, platelet aggregation, and chemotaxis. Edg2 is involved in myelin formation or maintenance. It is activated by platelet-derived lysophosphatidic acid (LPA), a lipid metabolite present in serum that acts ubiquitously. Although alternate translation start codons and polyadenylation sites have been reported, the alternative splicing transcripts each encode identical proteins. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate, and uterus libraries.

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Entrez Gene IDs

1902 (Human)

Gene Symbol

LPAR1

UniProt

Product Documents for LPAR1/LPA1/EDG-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LPAR1/LPA1/EDG-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for LPAR1/LPA1/EDG-2 Antibody - BSA Free (NLS212):

Immunohistochemistry Protocol for EDG2 Antibody (NLS212): https://www.novusbio.com/products/lpar1-lpa1-edg-2-antibody_nls212
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.
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