LOX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2530
![Western Blot: LOX AntibodyBSA Free [NB100-2530] Western Blot: LOX AntibodyBSA Free [NB100-2530]](https://resources.bio-techne.com/images/products/LOX-Antibody-Western-Blot-NB100-2530-img0006.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine
Cited:
Human, Mouse
Predicted:
Chicken (92%), Xenopus (92%), Zebrafish (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A cocktail of two synthetic peptides; one made to a region of the human LOX protein within residues 300-350 (NB100-2528) and one within residues 200-300 (NB100-2527). [Swiss-Prot P28300]
Reactivity Notes
92% sequence identity with chicken, Zebrafish, and Xenopus proteins.
Localization
Cytosolic and nuclear.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LOX Antibody - BSA Free
Western Blot: LOX AntibodyBSA Free [NB100-2530]
Western Blot: LOX Antibody [NB100-2530] - Detection of LOX in mouse kidney lysate using NB 100-2530 (1:1,000). ECL detection was 15 sec.Immunocytochemistry/ Immunofluorescence: LOX Antibody - BSA Free [NB100-2530]
Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2530] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LOX [NB100-2530] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: LOX Antibody - BSA Free [NB100-2530]
LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2530-img0010.jpgApplications for LOX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Simple Western
1:100
Western Blot
1:500-1:1000
Application Notes
This LOX antibody is useful for Western blot and Immunohistochemistry paraffin embedded sections. In Western blot a band is seen at ~58 kDa. This is a highly glycosylated protein; therefore one may see bands at ~32 kDa, ~50 kDa and ~58 kDa representing the mature (secreted) form, the pro form and the glycosylated forms respectively.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 57 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 57 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Lysyl Oxidase/LOX
Long Name
Lysyl Oxidase
Alternate Names
AAT10
Gene Symbol
LOX
UniProt
Additional Lysyl Oxidase/LOX Products
Product Documents for LOX Antibody - BSA Free
Product Specific Notices for LOX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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