LOX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2527
![Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0011.jpg "Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]")
![Western Blot: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Western-Blot-NB100-2527-img0015.jpg "Western Blot: LOX Antibody [NB100-2527]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human, Mouse, Rat, Porcine, Feline
Predicted:
Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal region of the human LOX protein (within residues 200-300). [Swiss-Prot P28300].
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 27169768). Immunogen displays the following percentage of sequence identity for non-tested species: bovine (92%), chicken (92%), xenopus (85%). Porcine reactivity reported in scientific literature (PMID: 30036726).
Localization
Secreted, extracellular space.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LOX Antibody - BSA Free
![Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0017.jpg "Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]")
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]
LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0017.jpg![Simple Western: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Simple-Western-NB100-2527-img0009.jpg "Simple Western: LOX Antibody [NB100-2527]")
Simple Western: LOX Antibody [NB100-2527]
Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Knockdown Validated: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Knockdown-Validated-NB100-2527-img0016.jpg "Western Blot LOX Antibody [NB100-2527]")
![Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2527-img0007.jpg "Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]")
![Immunohistochemistry: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunohistochemistry-NB100-2527-img0014.jpg "Immunohistochemistry: LOX Antibody [NB100-2527]")
![Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2527-img0013.jpg "Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]")
![Western Blot: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Western-Blot-NB100-2527-img0005.jpg "Western Blot: LOX Antibody [NB100-2527]")
![Immunohistochemistry: LOX Antibody [NB100-2527]](https://resources.bio-techne.com/images/products/LOX-Antibody-Immunohistochemistry-NB100-2527-img0006.jpg "Immunohistochemistry: LOX Antibody [NB100-2527]")
Applications for LOX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100-1:250
Immunohistochemistry-Paraffin
1:100-1:250
Simple Western
1:25
Western Blot
0.5-4ug/ml
Application Notes
This LOX antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western blot. In Western blot, bands are observed ~58 kDa representing glycosylated Lox and ~32 kDa representing the mature, secreted form of Lox. In ICC/IF nuclear staining was observed in HeLa cells, which is expected for the mature form of LOX according to published literature (PMID 17287363 and 10996848).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 27 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 27 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 5 reviews rated 4 using NB100-2527 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Lysyl Oxidase/LOX
Long Name
Lysyl Oxidase
Alternate Names
AAT10
Gene Symbol
LOX
Additional Lysyl Oxidase/LOX Products
Product Documents for LOX Antibody - BSA Free
Product Specific Notices for LOX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...