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LMW-PTP/ACP1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99671

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human LMW-PTP/ACP1 (Accession#: AAI06012.1; Met1-His158)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LMW-PTP/ACP1 Antibody - Azide and BSA Free

Western Blot: LMW-PTP/ACP1 Antibody [NBP2-99671]

Western Blot: LMW-PTP/ACP1 Antibody [NBP2-99671]

Western Blot: LMW-PTP/ACP1 Antibody [NBP2-99671] - Anti-LMW-PTP/ACP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa
Immunohistochemistry-Paraffin: LMW-PTP/ACP1 Antibody [NBP2-99671]

Immunohistochemistry-Paraffin: LMW-PTP/ACP1 Antibody [NBP2-99671]

Immunohistochemistry-Paraffin: LMW-PTP/ACP1 Antibody [NBP2-99671] - Immunochemical staining of human LMW-PTP/ACP1 in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: LMW-PTP/ACP1 Antibody [NBP2-99671]

Immunoprecipitation: LMW-PTP/ACP1 Antibody [NBP2-99671]

Immunoprecipitation: LMW-PTP/ACP1 Antibody [NBP2-99671] - LMW-PTP/ACP1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-LMW-PTP/ACP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LMW-PTP/ACP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa

Applications for LMW-PTP/ACP1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:1000-1:4000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMW-PTP/ACP1

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Alternate Names

ACP1, HAAP, LMWPTP

Gene Symbol

ACP1

Additional LMW-PTP/ACP1 Products

Product Documents for LMW-PTP/ACP1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LMW-PTP/ACP1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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