LIMPII/SR-B2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB400-129

Novus Biologicals, part of Bio-Techne
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NB400-129SS
NB400-129
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all LIMPII/SR-B2 Antibodies »

Product Specifications

Immunogen

A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]

Localization

Lysosome membrane; Multi-pass membrane protein.

Marker

Lysosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit LIMPII/SR-B2 Antibody - BSA Free (NB400-129) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-LIMPII/SR-B2 Antibody: Cited in 26 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LIMPII/SR-B2 Antibody - BSA Free

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

LIMPII-SR-B2-Antibody-Immunohistochemistry-Paraffin-NB400-129-img0009.jpg
Western Blot: LIMPII/SR-B2 Antibody [NB400-129]

Western Blot: LIMPII/SR-B2 Antibody [NB400-129]

Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).
Simple Western: LIMPII/SR-B2 Antibody [NB400-129]

Simple Western: LIMPII/SR-B2 Antibody [NB400-129]

Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.
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Applications for LIMPII/SR-B2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 23863627)

Immunoprecipitation

1:100

Western Blot

1:1000
Application Notes
In Western Blot, a band is observed at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB400-129 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIMPII/SR-B2

LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Entrez Gene IDs

950 (Human); 12492 (Mouse)

Gene Symbol

SCARB2

Additional LIMPII/SR-B2 Products

Product Documents for LIMPII/SR-B2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIMPII/SR-B2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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