LEUTX Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LEUTX Antibody - BSA Free [NBP1-90890] -

LEUTX expression in human preimplantation embryos and pluripotent stem cells. (A) Indirect immunolabeling in nuclei (blue) indicates LEUTX expression (red) in the human 8-cell embryo (n=3). Scale bar: 20 um. (B) STRT sequencing of single cells from human 8-cell blastomeres (n=14) and two hESC lines (HS983a and HS980, n=15 for each). The TSS-specific reads from LEUTX.n show low or undetectable expression in hESCs, but detectable expression in 8-cell blastomeres. ND, not detected. (C) qPCR validation in three hESC lines and an independent 8-cell embryo library. Expression was detected in all studied cell lines (n=3), but only in one replicate for HS980. (D-F) LEUTX expression in Yan et al. (2013), Xue et al. (2013) and Petropoulos et al. (2016) data, all supporting LEUTX expression in 4- and 8-cell embryos and downregulation in the morula/blastocyst. (F) Day 3 to day 7 refer to embryos from the 8-cell stage to late blastocyst. (G) FANTOM5 data for LEUTX (n=1829). Only six iPSC samples contained tag clusters in the LEUTX region. The average expression refers to the FANTOM5 CAGE phase 1 and 2 samples in which expression was detected. (H) Human somatic fibroblasts from amniotic mesoderm (n=3) were used to generate human iPSCs (hiPS, n=15) (GEO dataset GSE20750, probe 42402). LEUTX shows higher expression in human iPSCs. (I) hESCs (n=4) were differentiated into cardiomyocytes (n=4) and analyzed by RNA expression array (GEO dataset GSE13834, probe 42402). LEUTX was expressed in hESCs, but not in differentiated cardiomyocytes. LEUTX is detected by Agilent 014850 Whole Human Genome Microarray 4x44K G4112F (H,I). Error bars indicate mean+/-s.e.m.; all comparisons for G-I are statistically significant (Student's t-test, P=0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27578796), licensed under a CC-BY license. Not internally tested by Novus Biologicals.