Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44408

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3183

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Laminin alpha 4 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q16363, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free (NBP2-42392) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Analysis in human placenta and lymph node tissues using NBP3-44408 antibody. Corresponding Laminin alpha 4 RNA-seq data are presented for the same tissues.
Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Staining of human colon shows strong positivity in basement membrane of glandular cells.
Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44408]

Staining of human lymph node shows no positivity in non-germinal center cells as expected.
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Applications for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 4

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human laminin alpha-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.

Alternate Names

LAMA4

Gene Symbol

LAMA4

Additional Laminin alpha 4 Products

Product Documents for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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