Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 546215

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Laminin alpha 3/Laminin-5 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Laminin alpha3/Laminin‑5
aa 21-1713
Accession # NP_000218

Specificity

Detects human Laminin alpha3/Laminin‑5 in direct ELISAs and Western blots. In direct ELISAs, no cross reactivity with recombinant human (rh) Laminin  alpha1, beta1, gamma2, or recombinant mouse Laminin  alpha4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Laminin alpha 3/Laminin-5 Antibody (546215) [FITC]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Laminin alpha 3/Laminin-5

The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Gene Symbol

LAMA3

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Laminin alpha 3/Laminin-5 Antibody (546215) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Laminin alpha 3/Laminin-5 Antibody (546215) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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