Laminin alpha 1 Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF4187CL1
![Laminin alpha 1 Antibody [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/af4187cl1_goat-laminin-alpha-1-pab-corafluor-1-109202511353220.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry
Label
CoraFluor 1
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant human Laminin alpha1 N-Terminus Domain VI
Leu22-Met269
Accession # P25391
Leu22-Met269
Accession # P25391
Specificity
Detects human Laminin alpha1 N-Terminus Domain VI in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAMA4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Laminin alpha 1 Antibody [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Laminin alpha 1 Antibody [CoraFluor™ 1]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Laminin alpha 1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).
Alternate Names
LAMA1
Gene Symbol
LAMA1
Additional Laminin alpha 1 Products
Product Documents for Laminin alpha 1 Antibody [CoraFluor™ 1]
Product Specific Notices for Laminin alpha 1 Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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