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Lamin B1 Antibody (23GB915)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50902

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NBP3-50902-100ul
NBP3-50902-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB915

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Lamin B1 (Uniprot: P20700)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin B1 Antibody (23GB915)

Lamin B1 Antibody (23GB915)

Immunocytochemistry/ Immunofluorescence: Lamin B1 Antibody (23GB915)[NBP3-50902] -

Immunocytochemical staining of HepG2 cells with Lamin B1 antibody (1:1,000). Nuclei were stained blue with DAPI; Lamin B1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Lamin B1 Antibody (23GB915)

Flow Cytometry: Lamin B1 Antibody (23GB915)[NBP3-50902] -

Flow cytometric analysis of Lamin B1expression in C2C12 cells using Lamin B1 antibody (isotype control; red, Lamin B1.
Lamin B1 Antibody (23GB915)

Western Blot: Lamin B1 Antibody (23GB915)[NBP3-50902] -

Western blotting analysis using Lamin B1 antibody. Total cell lysates (30 ug) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lamin B1 antibody and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Lamin B1 Antibody (23GB915)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (23GB915)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lamin B1 Antibody (23GB915)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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