KRAS Antibody (D113) - Mutant - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43583
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # D113
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'. (Uniprot# P01116-2)
Specificity
This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37% pancreatic cancers and over 12% of colorectal cancers.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KRAS Antibody (D113) - Mutant - BSA Free
Immunocytochemistry /Immunofluorescence: KRAS Antibody (D113) - Mutant - BSA Free [NBP3-43583] -
Immunocytochemistry /Immunofluorescence: KRAS Antibody (D113) - Mutant - BSA Free [NBP3-43583] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells on Shi-fix coverslips stained with NBP3-43583 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43583, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Applications for KRAS Antibody (D113) - Mutant - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KRAS
Alternate Names
cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, Ki-Ras, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Symbol
KRAS
Additional KRAS Products
Product Documents for KRAS Antibody (D113) - Mutant - BSA Free
Product Specific Notices for KRAS Antibody (D113) - Mutant - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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