KLF8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57740
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PALFNDIKIEPPEELLASDFSLPQVEPVDLSFHKPKAPLQPASMLQAPIRPPKPQSSPQ
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31827393).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KLF8 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: KLF8 Antibody [NBP2-57740]
Immunocytochemistry/Immunofluorescence: KLF8 Antibody [NBP2-57740] - Staining of human cell line RT4 shows localization to nucleoplasm & aggresome.Immunocytochemistry/ Immunofluorescence: KLF8 Antibody - BSA Free [NBP2-57740] -
DANCR knockdown blocked the tumor formation in vivo involving KLF8 activation. CAL-27 or TCa-8113 cells transfected with shRNA against DANCR were inoculated subcutaneously into the nude mice. Xenografts were measured every 4 days with a caliper. a Tumor volumes were measured every 4 days. b Mice were sacrificed after 25 days, and xenograft tumors were excised and weighed. c Relative expression of MMP-2 and MMP-9 protein in tumor tissues was measured by western blot after 25 days. d Relative expression of KLF8 protein in tumor tissues was examined by western blot after 25 days. e Immunofluorescence staining was performed to investigate KLF8 immunoreactive materials in tumor tissues after 25 days. ***p < 0.001, versus to sh-NC Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for KLF8 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Paraffin
Use in IHC-P reported in scientific literature (PMID:31827393).
Western Blot
Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31827393), licensed under a CC-BY licence.
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF8
Alternate Names
Basic krueppel-like factor 3, basic kruppel-like factor 3, BKLF3DKFZp686O08126, DXS741, Krueppel-like factor 8, Kruppel-like factor 8, Zinc finger protein 741, ZNF741MGC138314
Gene Symbol
KLF8
Additional KLF8 Products
Product Documents for KLF8 Antibody - BSA Free
Product Specific Notices for KLF8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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