KLF4 Antibody (CL5782) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57889
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # CL5782
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from O43474, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for KLF4 Antibody (CL5782) - Azide and BSA Free
KLF4 Antibody (CL5782) - BSA Free
Analysis in human cell line U-2197.
KLF4 Antibody (CL5782) - BSA Free
Analysis in human cell line U-2197.
KLF4 Antibody (CL5782) - BSA Free
Staining of human placenta shows nuclear positivity in the trophoblast.
Applications for KLF4 Antibody (CL5782) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF4
Long Name
Kruppel-Like Factor 4
Alternate Names
EZF
Gene Symbol
KLF4
Additional KLF4 Products
Product Documents for KLF4 Antibody (CL5782) - Azide and BSA Free
Product Specific Notices for KLF4 Antibody (CL5782) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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