Kirrel1/NEPH1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03509
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-200 of human Kirrel1/NEPH1 (NP_060710.3). QGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Kirrel1/NEPH1 Antibody - Azide and BSA Free (NBP3-03509) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Kirrel1/NEPH1 Antibody - Azide and BSA Free
![Western Blot: Kirrel1/NEPH1 AntibodyAzide and BSA Free [NBP3-03509]](https://resources.bio-techne.com/images/products/Kirrel1-NEPH1-Antibody-Western-Blot-NBP3-03509-img0001.jpg "Western Blot: Kirrel1/NEPH1 AntibodyAzide and BSA Free [NBP3-03509]")
Western Blot: Kirrel1/NEPH1 AntibodyAzide and BSA Free [NBP3-03509]
Western Blot: Kirrel1/NEPH1 Antibody [NBP3-03509] - Analysis of extracts of various cell lines, using Kirrel1/NEPH1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Applications for Kirrel1/NEPH1 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kirrel1/NEPH1
Kirrel (kin of irregular chiasm-1-like) proteins, also known as the NEPH (nephrin-like) family, are type I transmembrane glycoproteins within the immunoglobulin superfamily. Kirrel1 (NEPH1), Kirrel2 (NEPH3, Filtrin, NLG-1) and Kirrel3 (NEPH2, kirre) share 34 - 44% amino acid (aa) identity, and all interact with nephrin, podocin and the adaptor molecule ZO-1 (1, 4). All can be found in podocytes of the kidney glomerulus, and play a role in barrier function in the slit diaphragm. (2-6). Kirrel1 can also be found in central nervous system neurons, pancreas and placenta, Kirrel2 in pancreatic islet beta cells, brain and lymph nodes, and Kirrel3 in the brain and bone marrow, typically at sites where nephrin is also expressed.
Long Name
Kin of Irregular Chiasm-like 1
Alternate Names
KIRREL, NEPH1
Gene Symbol
KIRREL1
Additional Kirrel1/NEPH1 Products
Product Documents for Kirrel1/NEPH1 Antibody - Azide and BSA Free
Product Specific Notices for Kirrel1/NEPH1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov