Ki67/MKI67 Antibody [DyLight 594]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-170DL594

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NB500-170DL594
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NB500-170
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Avian

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Ki67/MKI67 Antibodies »

Product Specifications

Immunogen

The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, between amino acids: 1550-1600 .

Reactivity Notes

Use in Avian reported in scientific literature (PMID:34986451) Ki67/MKI67 Antibody reacted with Mouse (PMID: 22033079), Rat (PMID: 22521325), and Porcine (PMID: 29146772). .

Localization

Nuclear

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Ki67/MKI67 Antibody [DyLight 594]

Western Blot: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Western Blot: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Western Blot: Ki-67/MKI67 Antibody [NB500-170DL594] - A431 (A) and Hek293 (B) cell lysate using Ki67 antibody (NB500-170) at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Immunocytochemistry/Immunofluorescence: Ki-67/MKI67 Antibody [NB500-170DL594] - Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [DyLight 594] [NB500-170DL594]

Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170DL594] - Human tonsil.
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Applications for Ki67/MKI67 Antibody [DyLight 594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB500-170. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.005% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126, antiKi67, Ki67 ihc, Ki-67 ihc, Ki67 mouse, Ki-67 mouse, Ki67 western blot, Ki-67 western blot

Entrez Gene IDs

4288 (Human); 17345 (Mouse); 291234 (Rat)

Gene Symbol

MKI67

UniProt

P46013 (Human), Q91VE6 (Mouse)

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody [DyLight 594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ki67/MKI67 Antibody [DyLight 594]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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