Ki67/MKI67 Antibody (6P3K5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57376

Recombinant Monoclonal Antibody
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NBP3-57376-100ul
NBP3-57376-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6P3K5

View all Ki67/MKI67 Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# P46013)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Pancreas, Lung, Tonsil, Cervix, Placenta, Colon, Kidney, Spleen, Liver, esophagus

Cancer Tissues Tested
Breast cancer, Colon cancer, Thyroid cancer, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Lung adenocarcinoma, Lung squamous carcinoma

Positive Controls
Preferentially during late G1, S, G2 and M phases of the cell cycle tonsil (A moderate to strong, distinct nuclear staining reaction in 80-90% of the germinal centre B-cells in both the light and the dark zone and in the vast majority of the suprabasal squamous epithelial cells), esophagus (squamous epithelia)

Negative Controls
Liver (Hepatocytes, <1%)

Applications for Ki67/MKI67 Antibody (6P3K5)

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Frozen

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Multiplex Immunoassay

1:200 - 1:800

Western Blot

1:2000 - 1:10000
Application Notes
This product can be used in Multiplex Immunohistochemistry at a recommended concentration of 1:200 - 1:800.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody (6P3K5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ki67/MKI67 Antibody (6P3K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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