Keratin 31 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48496
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Keratin 31. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Keratin 31 Antibody (NBP3-48496) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Keratin 31 Antibody
Immunocytochemistry/ Immunofluorescence: Keratin 31 Antibody [NBP3-48496] -
Keratin 31 antibody [N3C3] detects KRT31 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KRT31 protein stained by Keratin 31 antibody [N3C3] (NBP3-48496) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemistry: Keratin 31 Antibody [NBP3-48496] -
Immunohistochemical analysis of paraffin-embedded Gastric ca, using Keratin 31(NBP3-48496) antibody at 1:500 dilution.Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Western Blot: Keratin 31 Antibody [NBP3-48496] -
Sample (30 ug of whole cell lysate)A: HeLa
10% SDS PAGE
NBP3-48496 diluted at 1:5000
Applications for Keratin 31 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Keratin 31
Alternate Names
Hair Keratin, Type I Ha, Hard Keratin, Type I, 1, hHa1, K31, Keratin 31, Keratin, Hair, Acidic, 1, Keratin, Type I Cuticular Ha1, Keratin-31, KRTHA1
Gene Symbol
KRT31
Additional Keratin 31 Products
Product Documents for Keratin 31 Antibody
Product Specific Notices for Keratin 31 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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