KAT3B/p300 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-161
![Western Blot: KAT3B/p300 Antibody [NB500-161]](https://resources.bio-techne.com/images/products/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161]")
![Western Blot: KAT3B/p300 Antibody [NB500-161]](https://resources.bio-techne.com/images/products/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0012.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Immunoprecipitation, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).
Reactivity Notes
Rat reactivity reported in scientific literature (PMID:33106900).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KAT3B/p300 Antibody - BSA Free
Western Blot: KAT3B/p300 Antibody [NB500-161]
KAT3B-p300-Antibody-Western-Blot-NB500-161-img0012.jpg![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161]](https://resources.bio-techne.com/images/products/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg "Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161]")
Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161]
Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB![Western Blot: KAT3B/p300 Antibody [NB500-161]](https://resources.bio-techne.com/images/products/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161]")
Western Blot: KAT3B/p300 Antibody [NB500-161]
Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).Applications for KAT3B/p300 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunohistochemistry
1:500 to 1:2000
Immunohistochemistry-Paraffin
1:500 to 1:2000
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAT3B/p300 antibody validated for chip from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NB500-161 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p300
Long Name
E1A-binding Protein p300
Alternate Names
EP300
Entrez Gene IDs
2033 (Human)
Gene Symbol
EP300
UniProt
Additional p300 Products
Product Documents for KAT3B/p300 Antibody - BSA Free
Product Specific Notices for KAT3B/p300 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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