JNK/JIP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1246

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NB100-1246
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all JNK/JIP3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence MEIQMDEGGGVV-C corresponding to N-Terminus according to NP_055948.2, NP_001035529.1.

Specificity

This antibody is expected to recognise both reported Human isoforms of this protein (as represented by NP_055948.2 and NP_001035529.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for JNK/JIP3 Antibody

Immunohistochemistry-Paraffin: JNK/JIP3 Antibody [NB100-1246]

Immunohistochemistry-Paraffin: JNK/JIP3 Antibody [NB100-1246]

Immunohistochemistry-Paraffin: JNK/JIP3 Antibody [NB100-1246] - (4ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for JNK/JIP3 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4 - 6 ug/ml

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: No signal obtained yet but low background observed in human heart lysate at up to 2ug/ml. IHC-P: Mouse brain shows axonal staining in various regions.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK/JIP3

The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

C-jun-amino-terminal kinase interacting protein 3, C-Jun-amino-terminal kinase-interacting protein 3, DKFZp762N1113, homolog of Drosophila Sunday driver 2, JIP-3, JIP3FLJ00027, JNK MAP kinase scaffold protein 3, JNK/SAPK-associated protein-1, JNK/stress-activated protein kinase-associated protein 1, JNK-interacting protein 3, JSAP1, KIAA1066SYD2, mitogen-activated protein kinase 8 interacting protein 3, Mitogen-activated protein kinase 8-interacting protein 3, syd

Entrez Gene IDs

23162 (Human); 30957 (Mouse)

Gene Symbol

MAPK8IP3

UniProt

NP_055948 (Human)

Additional JNK/JIP3 Products

Product Documents for JNK/JIP3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JNK/JIP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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