JAM-A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49908

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NBP1-49908
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all JAM-A Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 249 and 299 of human Junctional Adhesion Molecule A using the numbering given in entry NP_058642.1 (GeneID 50848).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34293456)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JAM-A Antibody

Western Blot: JAM-A Antibody [NBP1-49908]

Western Blot: JAM-A Antibody [NBP1-49908]

Western Blot: JAM-A Antibody [NBP1-49908] - Samples: Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody NBP1-49908 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. JAM-A was not successfully immunoprecipitated by rabbit anti-JAM-A antibody NBP1-49907, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Immunohistochemistry-Paraffin: JAM-A Antibody [NBP1-49908]

Immunohistochemistry-Paraffin: JAM-A Antibody [NBP1-49908]

Immunohistochemistry-Paraffin: JAM-A Antibody [NBP1-49908] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for JAM-A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: JAM-A

Junctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. JAM is selectively concentrated at intercellular junctions of endothelial and epithelial cells of different origins. Microscopy shows that JAM codistributes with tight junction components at the apical region of the intercellular cleft. A mAb directed to JAM (BV11) was found to inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer in vitro. Systemic treatment of mice with BV11 mAb blocked monocyte infiltration upon chemokine administration in subcutaneous air pouches (1). The human protein shares a highly conserved structure and sequence with the murine protein. However it is distinct in that it is constitutively expressed on circulating neutrophils, monocytes, platelets and lymphocyte subsets (2).

Long Name

Junctional Adhesion Molecule A

Alternate Names

CD321, F11R, JAMA, PAM-1

Gene Symbol

F11R

Additional JAM-A Products

Product Documents for JAM-A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JAM-A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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