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IRF2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56712

Novus Biologicals, part of Bio-Techne
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NBP2-56712
NBP2-56712-25ul

Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation-exo-Seq, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SMSELYPLQISPVSSYAESETTDSVPSDEESAEGRPHWRKRNIEGKQYLSNMGTRGSYLLPGMASFVTSNKPDLQVTIKEESNPVPYNSSWPPFQDLPLSSSMTPASSSSRPDRETRASVIKKTSD

Reactivity Notes

Mouse 87%, Rat 85%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF2 Antibody - BSA Free

Western Blot: IRF2 Antibody [NBP2-56712]

Western Blot: IRF2 Antibody [NBP2-56712]

Western Blot: IRF2 Antibody [NBP2-56712] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: IRF2 Antibody [NBP2-56712]

Immunocytochemistry/ Immunofluorescence: IRF2 Antibody [NBP2-56712]

Immunocytochemistry/Immunofluorescence: IRF2 Antibody [NBP2-56712] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
IRF2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: IRF2 Antibody - BSA Free [NBP2-56712]

Chromatin Immunoprecipitation-exo-Seq: IRF2 Antibody - BSA Free [NBP2-56712]

ChIP-Exo-Seq composite graph for Anti-IRF2 (NBP2-56712) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for IRF2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF2

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.

Long Name

Interferon Regulatory Factor 2

Alternate Names

DKFZp686F0244, interferon regulatory factor 2, IRF-2

Gene Symbol

IRF2

Additional IRF2 Products

Product Documents for IRF2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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