I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-62660

I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62660

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Scientific Data
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNE

Reactivity Notes

Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin beta 1/CD29 Antibody - BSA Free

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660] - Analysis in human smooth muscle and pancreas tissues using Anti-ITGB1 antibody. Corresponding ITGB1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660] - Staining of human smooth muscle shows high expression.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660] - Staining of human pancreas shows low expression as expected.

Applications for Integrin beta 1/CD29 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody - BSA Free

Product Datasheets

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COA

SDS

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Product Specific Notices for Integrin beta 1/CD29 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Integrin beta 1/CD29 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62660

Key Product Details

Validated by

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Format

Concentration

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for Integrin beta 1/CD29 Antibody - BSA Free

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62660]

Applications for Integrin beta 1/CD29 Antibody - BSA Free

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin beta 1/CD29

Alternate Names

Gene Symbol

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Integrin beta 1/CD29 Antibody - BSA Free