Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50449
![Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]](https://resources.bio-techne.com/images/products/Integrin-alpha-V-+-beta-6-Antibody-AvB6-53a-2-Western-Blot-NBP2-50449-img0002.jpg "Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # AvB6 53a.2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Mouse fibroblasts retrovirally infected with cDNA to human Integrin alpha V beta 6 (3T3b6.19). Cells expressed a mouse/human Integrin alpha V beta 6 chimera.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Description
Novus Biologicals Rat Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free (NBP2-50449) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. Anti-Integrin alpha V beta 6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]
Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) [NBP2-50449] - avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds, and 53a2 shows only the upper band of B6. By 1 minute, most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.Applications for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Application Notes
Positive control(s): BT20 breast carcinoma, original immunogen. This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin alpha V beta 6
Integrin alpha V beta 6 (aVb6) is a heterodimeric adhesion protein that is exclusively expressed on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas. Active aVb6 binds to the extracellular matrix proteins Fibronectin and Tenascin C as well as the TGF-beta latency-associated peptide (LAP). It cooperates with LTBP-1, Fibronectin, and PAR-1 to activate TGF-beta 1 or TGF-beta 3 from large latent complexes. aVb6 also plays a role in Treg development, tumor progression, and virus cellular entry.
Alternate Names
CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR
Gene Symbol
ITGAV
Additional Integrin alpha V beta 6 Products
Product Documents for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Product Specific Notices for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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