Integrin alpha 5/CD49e Antibody (CL6951) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76520

Novus Biologicals, part of Bio-Techne
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NBP2-76520-25ul
NBP2-76520
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-44404AF350
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Alexa Fluor 405
NBP3-44404AF405
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Alexa Fluor 488
NBP3-44404AF488
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Alexa Fluor 532
NBP3-44404AF532
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Alexa Fluor 594
NBP3-44404AF594
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Alexa Fluor 647
NBP3-44404AF647
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Alexa Fluor 700
NBP3-44404AF700
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Alexa Fluor 750
NBP3-44404AF750
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Alexa Fluor Plus 488
NBP3-44404AFP488
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Alexa Fluor Plus 555
NBP3-44404AFP555
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Alexa Fluor Plus 594
NBP3-44404AFP594
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Alexa Fluor Plus 647
NBP3-44404AFP647
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Alexa Fluor Plus 680
NBP3-44404AFP680
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Allophycocyanin
NBP3-44404APC
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Biotin
NBP3-44404B
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CoraFluor 1
NBP3-44404CL1
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DyLight 350
NBP3-44404UV
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DyLight 405
NBP3-44404V
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DyLight 488
NBP3-44404G
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DyLight 550
NBP3-44404R
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DyLight 594
NBP3-44404DL594
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DyLight 650
NBP3-44404C
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DyLight 680
NBP3-44404FR
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DyLight 755
NBP3-44404IR
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FITC
NBP3-44404F
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HRP
NBP3-44404H
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Janelia Fluor 525
NBP3-44404JF525
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Janelia Fluor 549
NBP3-44404JF549
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Janelia Fluor 646
NBP3-44404JF646
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Janelia Fluor 669
NBP3-44404JF669
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mFluor Violet 450 SE
NBP3-44404MFV450
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mFluor Violet 500 SE
NBP3-44404MFV500
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mFluor Violet 610 SE
NBP3-44404MFV610
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PE
NBP3-44404PE
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PerCP
NBP3-44404PCP
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL6951

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha 5/CD49e Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P08648, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85)% and Rat (82)%

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Integrin alpha 5/CD49e Antibody (CL6951) - BSA Free

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520]

Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human cell line U-87 MG.
View 4 more images

Applications for Integrin alpha 5/CD49e Antibody (CL6951) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (CL6951) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 5/CD49e Antibody (CL6951) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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