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Integrin alpha 5/CD49e Antibody (23GB1050)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51959

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NBP3-51959-100ul
NBP3-51959-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1050

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Integrin alpha 5/CD49e (Uniprot: P08648)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha 5/CD49e Antibody (23GB1050)

Integrin alpha 5/CD49e Antibody (23GB1050)

Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (23GB1050)[NBP3-51959] -

Immunocytochemical staining of C2C12 cells with Integrin alpha 5/CD49e antibody (1:1,000). Nuclei were stained blue with DAPI; Integrin alpha 5/CD49e was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Integrin alpha 5/CD49e Antibody (23GB1050)

Flow Cytometry: Integrin alpha 5/CD49e Antibody (23GB1050)[NBP3-51959] -

Validation of Integrin alpha 5/CD49e knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Integrin alpha 5/CD49e antibody (1:2,000) and analyzed using CytoFLEX.
Integrin alpha 5/CD49e Antibody (23GB1050)

Flow Cytometry: Integrin alpha 5/CD49e Antibody (23GB1050)[NBP3-51959] -

Flow cytometric analysis of Integrin alpha 5/CD49eexpression in C2C12 cells using Integrin alpha 5/CD49e antibody (1:2,000). Green, isotype control; red, Integrin alpha 5.

Applications for Integrin alpha 5/CD49e Antibody (23GB1050)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (23GB1050)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Integrin alpha 5/CD49e Antibody (23GB1050)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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