ING2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05515

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ING2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MLGQQQQQLYSSAALLTGERSRLLTCYVQDYLECVESLPHDMQRNVSVLRELDNKYQETLKEIDDVYEKYKKEDDLNQKKRLQQLLQRAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ING2 Antibody - BSA Free (NBP3-05515) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ING2 Antibody - BSA Free

Western Blot: ING2 Antibody [NBP3-05515]

Western Blot: ING2 Antibody [NBP3-05515]

Western Blot: ING2 Antibody [NBP3-05515] - Analysis in control (vector only transfected HEK293T lysate) and ING2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.
Immunocytochemistry/ Immunofluorescence: ING2 Antibody [NBP3-05515]

Immunocytochemistry/ Immunofluorescence: ING2 Antibody [NBP3-05515]

Immunocytochemistry/Immunofluorescence: ING2 Antibody [NBP3-05515] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: ING2 Antibody [NBP3-05515]

Immunohistochemistry-Paraffin: ING2 Antibody [NBP3-05515]

Immunohistochemistry-Paraffin: ING2 Antibody [NBP3-05515] - Staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.
View 1 more images

Applications for ING2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ING2

ING2 is similar to ING1, a tumor suppressor protein that can bind TP53, inhibit cell growth, and induce apoptosis. This protein binds phosphoinositides (PtdInPs) at its plant homeodomain (PHD) domain and it acts as a PtdInPs receptor. PtdInPs binding regulates the ability of this protein to activate TP53 and TP53-dependent apoptosis.

Alternate Names

ING1L, p33ING2

Gene Symbol

ING2

Additional ING2 Products

Product Documents for ING2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ING2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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