Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76404

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-76404
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR031F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Indoleamine 2,3-dioxygenase/IDO Antibodies »

Product Specifications

Immunogen

Recombinant protein representing Human IDO1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) (NBP2-76404) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-), HEK293T, HDLM-2, Hep-G2, IDO2 over-expressing HEK, untransfected HEK, and IDO1 over-expresssing HEK lysate. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Vinculin.
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 (red) in FFPE skin carcinoma by IHC-IF. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] NBP2-76404. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE tonsil by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
View 3 more images

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 -1:500

Immunohistochemistry

1:100 - 1:500

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.