Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Rat IgG2B Clone # 187515

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Indian Hedgehog/Ihh Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Indian Hedgehog
Cys28-Gly202
Accession # P97812

Specificity

Detects mouse Indian Hedgehog (Ihh) in direct ELISAs and Western blots. This antibody is specific for the N-terminal peptide of mouse Ihh (amino acids (aa) 66‑240). In Western blots, this antibody does not cross-react with recombinant mouse (rm) Dhh (aa 23‑198 or aa 199‑396), rmIhh (aa 241‑449) and has less than 25% cross-reactivity with rhShh (aa 24‑197), or rmShh (aa 25‑198 or aa 199‑437).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Indian Hedgehog/Ihh Antibody (187515) [Janelia Fluor® 669]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB1705. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Indian Hedgehog/Ihh

Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).

Alternate Names

BDA1, HHG2, Ihh

Gene Symbol

IHH

Additional Indian Hedgehog/Ihh Products

Product Documents for Indian Hedgehog/Ihh Antibody (187515) [Janelia Fluor® 669]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Indian Hedgehog/Ihh Antibody (187515) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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