IL-3R alpha/CD123 Antibody (6H6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1185
![Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Cytometry-NB600-1185-img0007.jpg "Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
![Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Cytometry-NB600-1185-img0011.jpg "Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular)
Cited:
Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6H6
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Tissue / cell preparation. IL3R transfected COS cells.
Localization
Type I membrane protein.
Specificity
IL3RA (6H6)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for IL-3R alpha/CD123 Antibody (6H6) - BSA Free
Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the PE/Cy7 conjugate of NB600-1185. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were![Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Cytometry-NB600-1185-img0001.jpg "Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of TF-1 cells with Purified Mouse IgG1, K Iso Cntrl (open histogram) or Purified anti-human CD123 (6H6) (colored histogram) followed by PE Anti-Mouse IgG. Total viable cells were used for analysis![Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Cytometry-NB600-1185-img0006.jpg "Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.![Flow (Intracellular): IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Intracellular-NB600-1185-img0008.jpg "Flow (Intracellular): IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
![Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]](https://resources.bio-techne.com/images/products/IL-3-R-alpha-CD123-Antibody-6H6-Flow-Cytometry-NB600-1185-img0009.jpg "Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]")
Applications for IL-3R alpha/CD123 Antibody (6H6) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1:10 - 1:1000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation. WB was reported in the scientific literature (PMID:12576313).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IL-3R alpha
Long Name
Interleukin 3 Receptor alpha
Alternate Names
CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA
Entrez Gene IDs
3563 (Human)
Gene Symbol
IL3RA
Additional IL-3R alpha Products
Product Documents for IL-3R alpha/CD123 Antibody (6H6) - BSA Free
Product Specific Notices for IL-3R alpha/CD123 Antibody (6H6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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