IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32568

Novus Biologicals, part of Bio-Techne
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NBP1-32568
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-1ra/IL-1F3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL-1ra/IL-1F3/IL1RN. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (83%), Porcine (82%), Bovine (81%).

Localization

Isoform 1: Secreted, Isoform 2: Cytoplasm, Isoform 3: Cytoplasm, Isoform 4: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IL-1ra/IL-1F3/IL1RN Antibody - Azide Free (NBP1-32568) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-IL-1ra/IL-1F3/IL1RN Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Immunocytochemistry/ Immunofluorescence: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Immunocytochemistry/Immunofluorescence: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568]

Immunohistochemistry-Paraffin: IL-1ra/IL-1F3/IL1RN Antibody [NBP1-32568] - Paraffin-embedded PC3 xenograft, using antibody at 1:500 dilution.
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Applications for IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-1ra/IL-1F3

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

Additional IL-1ra/IL-1F3 Products

Product Documents for IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-1ra/IL-1F3/IL1RN Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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