Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry, Functional Assay

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 48110

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-12 p70 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12

Specificity

Detects mouse IL-12 in ELISAs and Western blots. Detects an epitope in the p35 subunit. In sandwich ELISAs, does not cross-react with recombinant mouse IL-12 p40.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for IL-12 p70/IL-12A Antibody (48110) [PE]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-12 p70

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for IL-12 p70/IL-12A Antibody (48110) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-12 p70/IL-12A Antibody (48110) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.