IL-12/IL-35 p35 Antibody (116219) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1171A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 116219
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant porcine IL-12 p35/p40 heterodimer
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053
Specificity
Detects the heterodimeric form of porcine IL‑12 (p35/p40) in direct ELISAs. Western blotting data indicates recognition of the IL-12/IL-35 p35 subunit. In direct ELISAs and Western blots, 20‑100% cross-reactivity with the disulfide‑linked heterodomeric forms of recombinant mouse IL‑12 and recombinant human IL-12 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for IL-12/IL-35 p35 Antibody (116219) [Allophycocyanin]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB1171. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-12/IL-35 p35
Long Name
Interleukin 12 p35
Alternate Names
IL-12A, IL35p35
Additional IL-12/IL-35 p35 Products
Product Documents for IL-12/IL-35 p35 Antibody (116219) [Allophycocyanin]
Product Specific Notices for IL-12/IL-35 p35 Antibody (116219) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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