Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Virus

Applications

Validated:

ELISA, ELISA Detection (Matched Antibody Pair), Enzyme-linked Immunosorbent Spot, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot

Cited:

ESPOT

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # JES3-12G8

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all IL-10 Antibodies »

Product Specifications

Immunogen

COS-expressed human IL-10

Specificity

Human/Viral/Cynomolgus IL-10

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for IL-10 Antibody (JES3-12G8) - Azide and BSA Free

ELISA: IL-10 Antibody (JES3-12G8) - Azide and BSA Free [NBP2-27577]

ELISA: IL-10 Antibody (JES3-12G8) - Azide and BSA Free [NBP2-27577]

ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.

Applications for IL-10 Antibody (JES3-12G8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000-1:4000

Immunohistochemistry

1:10 - 1:2000

Immunohistochemistry-Frozen

1:10 - 1:2000
Application Notes
May be paired with NBP2-77379 in a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

1.0 mL of borate buffered saline, pH 8.2.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

UniProt

NP_000563 (Human)

Additional IL-10 Products

Product Documents for IL-10 Antibody (JES3-12G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-10 Antibody (JES3-12G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.