IKK alpha Antibody (23GB1065)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51974

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NBP3-51974-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1065

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK alpha Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human IKK alpha (Uniprot: O15111)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK alpha Antibody (23GB1065)

IKK alpha Antibody (23GB1065)

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (23GB1065)[NBP3-51974] -

IKK alpha antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
IKK alpha Antibody (23GB1065)

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (23GB1065)[NBP3-51974] -

Immunocytochemical staining of HepG2 cells with IKK alpha antibody (1:1,000). Nuclei were stained blue with DAPI; Component of inhibitor of IKK alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar, 20 um.
IKK alpha Antibody (23GB1065)

Flow Cytometry: IKK alpha Antibody (23GB1065)[NBP3-51974] -

Validation of IKK alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IKK alpha antibody (1:2,000) and analyzed using BD flow cytometer.
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Applications for IKK alpha Antibody (23GB1065)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK alpha

IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Gene Symbol

CHUK

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (23GB1065)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK alpha Antibody (23GB1065)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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