IkB-beta Antibody (24GB235)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50516

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NBP3-50516-100ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB235

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-beta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human IkB-beta (Uniprot: Q15653)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-beta Antibody (24GB235)

IkB-beta Antibody (24GB235)

Immunocytochemistry/ Immunofluorescence: IkB-beta Antibody (24GB235)[NBP3-50516] -

Immunocytochemical staining of HepG2 cells with IkB-beta antibody (1:1,000). Nuclei were stained blue with DAPI; IkB-beta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
IkB-beta Antibody (24GB235)

Flow Cytometry: IkB-beta Antibody (24GB235)[NBP3-50516] -

Flow cytometric analysis of IkB-betaexpression in HepG2 cells using IkB-beta antibody (1:2,000). Green, isotype control; red, IKB beta.
IkB-beta Antibody (24GB235)

Western Blot: IkB-beta Antibody (24GB235)[NBP3-50516] -

Western blotting analysis using IkB-beta antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for IkB-beta Antibody (24GB235)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IkB-beta

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Long Name

I-kappa-B-beta

Alternate Names

IkBbeta, NFKBIB, TR-interacting protein 9

Gene Symbol

NFKBIB

Additional IkB-beta Products

Product Documents for IkB-beta Antibody (24GB235)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IkB-beta Antibody (24GB235)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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