IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56065
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine
Applications
Western Blot, ELISA
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 39A1413
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.
Modification
p Ser32, p Ser36
Specificity
IkBa (39A1413 ) [Ser32/Ser36]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin]
Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin] [NB100-56065] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56065(lanes 1 &2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56065 detects specifically the phosphorylated form of IkBa.
Applications for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin]
Application
Recommended Usage
ELISA
1:100-1:1000
Western Blot
1 ug/ml
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-56724. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IkB-alpha
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Gene Symbol
NFKBIA
Additional IkB-alpha Products
Product Documents for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin]
Product Specific Notices for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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