Ikaros/IKZF1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38242

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NBP2-38242
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Simple Western, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ikaros Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DADEGQDMSQVSGKESPPVSDTPDEGDEPMPIPEDLSTTSGGQQSSKSDRVVASNVKVETQSDEENGRACEMNGEECAEDLRMLDASGEKMNGSH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ikaros/IKZF1 Antibody - BSA Free

Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242]

Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242]

Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Ikaros/IKZF1 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: Ikaros/IKZF1 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: Ikaros/IKZF1 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-Ikaros/IKZF1 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.
Ikaros/IKZF1 Antibody - BSA Free Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242]

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242]

Analysis in human tonsil and cerebral cortex tissues. Corresponding RNA-seq data are presented for the same tissues.
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Applications for Ikaros/IKZF1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: RPMI 8266 cell line at 1.0 mg/ml as sample; separated by size; antibody dilution of 1:50; observed molecular weight was 35-77 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ikaros

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Long Name

DNA-binding protein Ikaros

Alternate Names

IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1

Gene Symbol

IKZF1

UniProt

Q13422

Additional Ikaros Products

Product Documents for Ikaros/IKZF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ikaros/IKZF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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