Ikaros/IKZF1 Antibody (4S10I3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16223
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4S10I3 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros/IKZF1 (Q13422). AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Ikaros/IKZF1 Antibody (4S10I3) (NBP3-16223) is a recombinant monoclonal antibody validated for use in WB, ELISA, ICC/IF and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Ikaros/IKZF1 Antibody (4S10I3)
![Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]](https://resources.bio-techne.com/images/products/Ikaros-IKZF1-Antibody-4S10I3-Western-Blot-NBP3-16223-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]")
Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]
Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] -
Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Immunofluorescence analysis of Jurkat cells using Ikaros/IKZF1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] -
Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros/IKZF1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for Ikaros/IKZF1 Antibody (4S10I3)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5μg antibody for 10μg-15μg of Chromatin
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ikaros
Long Name
DNA-binding protein Ikaros
Alternate Names
IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1
Gene Symbol
IKZF1
Additional Ikaros Products
Product Documents for Ikaros/IKZF1 Antibody (4S10I3)
Product Specific Notices for Ikaros/IKZF1 Antibody (4S10I3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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